Pharmacological assay showed that abi5-1 mutant was insensitive to TOR inhibitor AZD8055, whereas AtABI5 overexpression lines were hypersensitive to AZD8055 in Arabidopsis. Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 …

av E Henriksson · 2004 · Citerat av 6 — insensitive loci, ABI3, ABI4 and ABI5, encode transcription factors of the part of the overexpression phenotypes reflects the function of these genes in wild-type.

An ABI5 overexpression transgene was constructed by fusing an ABI5 cDNA into pGA643 (An et al., 1988) with 23 bp of 5′-UTR between the cauliflower mosaic virus 35S promoter and the initiating codon of ABI5. ABI5 was previously reported as the target protein of AFP2 during seed germination (Lopez-Molina et al., 2003), and overexpression of ABI5 activates the flowering negative factor FLC at the transcriptional level to repress flowering (Wang et al., 2013b). Thus, we compared the transcription of FLC in Col, afp2, and AFP2-ox under LD conditions. Loss-of-function in ABI5 (abi5) promotes juvenile-to-adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent. We demonstrate that FHY3 directly activates ABI5 expression and that overexpression of ABI5 rescues the seed germination defect of fhy3.

Abi5 overexpression

Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germi- nation. Bar, 1 cm. (D,E) Fresh weight and root length of abi5-1, ABI5-HA overexpression lines (OE) and WT seeds sown on plates containing DMSO, ABA (1 µM), and AZD (1 µM) for 10 days. Each graph 2016-12-20 Overexpression of ABI5 in plants to prevent precocious seed germination and to confer resistance to drought and high salt Mar 21, 2001 - The Rockefeller University The bZIP transcription factor ABI5 is shown to confer an enhanced response to exogenous abscisic acid during germination, seedling establishment and subsequent vegetative growth of plants.

ZFP3 overexpression in the Col-0, abi2-1, and abi4-. 101 backgrounds reduced hypocotyl lengths by. 50% to 60%, while in abi5-1/ZFP3ox seedlings, hy-.

Overexpression of ABI5 altered flowering time. (A) The expression level of ABI5-HA fusion protein in individual transgenic lines of p35S::ABI5-HA. Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ- accumulating (JAZ-DJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ Moreover, overexpression of ABI5 (ABI5-OE-8) in the JAZ8-ΔJas ABI3-OE background rendered the transgenic lines much more sensitive to ABA and COR during seed germination .

Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ- accumulating (JAZ-DJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ

By analogy to the related Novel Interactor of JAZ (NINJA) protein, it was suggested that the AFPs interact with the co-repressor TOPLESS to inhibit ABA-regulated gene expression. 2020-05-08 The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line. In sleepy1 mutant seeds, RGL2 overaccumulates; germination of these seeds can occur under conditions that produce low ABI5 … Overexpression of ABI5 in Col-0 influences flowering time To assess the correlation between flowering time and ABI5 expression, overexpressing transgenic lines carrying plas-mid p35S::ABI5-HA in Col-0 background were generated. A total of 43 lines were analysed by examining ABI5-HA protein level in individual transgenic plants (Fig. 1A). In the ABA-ABAR-WRKY40-ABI5 core signaling complex, WRKY40 acts to suppress ABA signaling, and high levels of ABA promote ABAR-WRKY40 interactions, then activate expression of ABA responsive genes, including the ABA responsive transcription factors ABI5 and ABI4.

(B) Phenotypes of abi5-1, ABI5 overexpression lines (OE) and WT seeds sown on plates containing DMSO, ABA (1 μM), and AZD (1 μM) for 14 days.
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The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line. Although it has been established that ABI5 delays ﬂowering by up-regulating FLC (Wang et al., 2013b), it is unknown whether and how AFPs affect ﬂowering time.Inthisstudy,wesoughttodeterminethepotential role of AFP2 in controlling ﬂowering time.

Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germination. Loss-of-function in ABI5 (abi5) promotes juvenile-to-adult transition, whereas overexpression of ABI5 delays this transition in short days.
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Loss‐of‐function in ABI5 (abi5) promotes juvenile‐to‐adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent.

2020-12-01 · Cross complementation experiments showed that overexpression of ABI5 could complements the ABA insensitive phenotype of abi3-1, whereas abi5-4 transgenic lines carrying a 35S-ABI3 transgene remained insensitive to ABA in seed germination, indicating that ABI5 acts downstream of ABI3 to reactivate late embryogenesis and to arrest the growth of germinating embryos (Lopez-Molina et al., 2002).